|
| Status |
Public on Mar 30, 2010 |
| Title |
NSC18 |
| Sample type |
RNA |
| |
|
| Source name |
kidney biopsy from patient after Tumor Nephrectomy
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: glomeruli of human kidney
disease state: nephrosclerosis
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Silica Gel column extraction of total RNA was performed according to the manufacturer's instructions.(Agilent Technologies, Waldbronn, Germany)Total RNA was isolated from microdissected glomeruli, reverse-transcribed, and linearly amplified.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Affymetrix Expression Analysis Technical Manual protocol was followed
|
| Scan protocol |
Affymetrix Expression Analysis Technical Manual protocol was followed
|
| Description |
Gene expression data from glomeruli of patient with Nephrosclerosis.
|
| Data processing |
RMA-Express was used for normalization and Genepattern and Genomatix software suites were used for statistical analysis
|
| |
|
| Submission date |
Mar 02, 2010 |
| Last update date |
Mar 16, 2010 |
| Contact name |
Preethi Sankaranarayanan |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Michigan
|
| Department |
Department of Internal Medicine - Division of Nephrology
|
| Lab |
Matthias Kretzler lab
|
| Street address |
1552 MSRB2,1150 W. Medical Center Drive
|
| City |
Ann Arbor |
| State/province |
MI |
| ZIP/Postal code |
48109 |
| Country |
USA |
| |
|
| Platform ID |
GPL96 |
| Series (1) |
| GSE20602 |
Human Nephrosclerosis Triggers a Hypoxia-Related Glomerulopathy |
|
