|
| Status |
Public on Mar 05, 2010 |
| Title |
SKBR3 control rep1 |
| Sample type |
RNA |
| |
|
| Source name |
SKBR3 cells transfected with siControl
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: SKBR3
transfection: siControl
cell type: breast cancer expressing mutant p53
|
| Treatment protocol |
SKBR3 cells were transfected with siControl or siChe-1 RNA double strand oligos and analyzed 48 hours later
|
| Growth protocol |
SKBR3 cells were cultured in RPMI 1640 medium with 10% FCS
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according manufacturer's instruction
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affimetrix protocol from 6 micrograms of total RNA (Gene Chip Whole Transcript Assay Technical Manual, 2007, Affimetrix)
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 micrograms of cRNA were hybridized for 16 hr at 45 °C on Human Gene 1. ST Array. GeneChips were whashed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affimetrix GeneChip Scanner 3000 7G system.
|
| Description |
SKBR3 control rep1
|
| Data processing |
Scanned image files (CEL) were processed, normalized (RMA-Sketch Quantile) and Log2-transformed by Expression Console Software (Affymetrix website)
|
| |
|
| Submission date |
Mar 04, 2010 |
| Last update date |
Mar 04, 2010 |
| Contact name |
maurizio fanciulli |
| E-mail(s) |
[email protected]
|
| Phone |
+390652662566
|
| Fax |
+390652662980
|
| Organization name |
regina elena cancer institute
|
| Department |
molecular medicine
|
| Lab |
epigenetics
|
| Street address |
via e chianesi 53
|
| City |
rome |
| ZIP/Postal code |
00144 |
| Country |
Italy |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE20622 |
Expression data from Che-1 (AATF) depleted SKBR3 and MDA-468 cell lines |
|
