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Sample GSM519982 Query DataSets for GSM519982
Status Public on Feb 29, 2012
Title OuJ, 24 hr Ozone, biological rep 1
Sample type RNA
 
Source name left lung lobe
Organism Mus musculus
Characteristics treatment group: 24 hr Ozone
tissue: left lung lobe
gender: Male
strain: C3H/ HeOuJ
Treatment protocol Animals were exposed to air or ozone, for 6, 24, 48 or 72 hours.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from individual lungs homogenized in TRIZOL. RNA was column-purified using the Qiagen RNeasy Mini Kit.
Label biotin
Label protocol 7.5 ug total RNA was processed according to the Affymetrix Technical manual Rev3 protocol. Biotinylated cRNA was prepared using the ENZO BioArray High Yield RNA Transcript Labeling Kit.
 
Hybridization protocol 10ug fragmented, biotinylated cRNA was hybridized to MOE430A GeneChips for 16hours at 45oC and 60rpm. The signal amplification protocol for washing/staining was performed in a FS450 automated fluidics station according to Affymetrix protocol.
Scan protocol GeneChips were scanned in an Affymetrix GCS3000 scanner with autoloader.
Description 3SRK 10-100
Data processing The data were analyzed with GCOS 1.4 software. All probe sets scaling was performed with a target signal value of 500. No normalization was performed.
 
Submission date Mar 09, 2010
Last update date Feb 29, 2012
Contact name Alison Kendall Bauer
E-mail(s) [email protected]
Organization name University of Colorado Denver
Department Environmental and Occupational Health
Street address 12850 E. Montview Blvd.; Rm 3125
City Aurora
State/province CO
ZIP/Postal code 80045
Country USA
 
Platform ID GPL339
Series (1)
GSE20715 Transcript analysis in response to ozone in mice deficient in TLR4

Data table header descriptions
ID_REF
VALUE GCOS 1.4 calculated signal intensity
ABS_CALL
DETECTION P-VALUE

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
1415670_at 912.8 P 0.000244
1415671_at 1743.4 P 0.000244
1415672_at 1835.1 P 0.000244
1415673_at 155.6 P 0.023926
1415674_a_at 695.4 P 0.000244
1415675_at 927.9 P 0.000732
1415676_a_at 2150.2 P 0.000732
1415677_at 442.3 P 0.008057
1415678_at 1932.5 P 0.000732
1415679_at 2682.6 P 0.000244
1415680_at 872.1 P 0.005859
1415681_at 598.3 P 0.000732
1415682_at 421.4 P 0.00293
1415683_at 1535.6 P 0.000244
1415684_at 357.2 P 0.000244
1415685_at 191.6 P 0.000244
1415686_at 1117.6 P 0.000244
1415687_a_at 6182.3 P 0.000244
1415688_at 1519.7 P 0.000244
1415689_s_at 623.8 P 0.001953

Total number of rows: 22690

Table truncated, full table size 621 Kbytes.




Supplementary file Size Download File type/resource
GSM519982.CEL.gz 2.1 Mb (ftp)(http) CEL
Processed data included within Sample table

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