|
| Status |
Public on Feb 29, 2012 |
| Title |
HeJ, 48 hr Ozone, biological rep 3 |
| Sample type |
RNA |
| |
|
| Source name |
left lung lobe
|
| Organism |
Mus musculus |
| Characteristics |
treatment group: 48 hr Ozone
tissue: left lung lobe
gender: Male
strain: C3H/HeJ
|
| Treatment protocol |
Animals were exposed to air or ozone, for 6, 24, 48 or 72 hours.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from individual lungs homogenized in TRIZOL. RNA was column-purified using the Qiagen RNeasy Mini Kit.
|
| Label |
biotin
|
| Label protocol |
7.5 ug total RNA was processed according to the Affymetrix Technical manual Rev3 protocol. Biotinylated cRNA was prepared using the ENZO BioArray High Yield RNA Transcript Labeling Kit.
|
| |
|
| Hybridization protocol |
10ug fragmented, biotinylated cRNA was hybridized to MOE430A GeneChips for 16hours at 45oC and 60rpm. The signal amplification protocol for washing/staining was performed in a FS450 automated fluidics station according to Affymetrix protocol.
|
| Scan protocol |
GeneChips were scanned in an Affymetrix GCS3000 scanner with autoloader.
|
| Description |
3SRK 13-14
|
| Data processing |
The data were analyzed with GCOS 1.4 software. All probe sets scaling was performed with a target signal value of 500. No normalization was performed.
|
| |
|
| Submission date |
Mar 09, 2010 |
| Last update date |
Feb 29, 2012 |
| Contact name |
Alison Kendall Bauer |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Colorado Denver
|
| Department |
Environmental and Occupational Health
|
| Street address |
12850 E. Montview Blvd.; Rm 3125
|
| City |
Aurora |
| State/province |
CO |
| ZIP/Postal code |
80045 |
| Country |
USA |
| |
|
| Platform ID |
GPL339 |
| Series (1) |
| GSE20715 |
Transcript analysis in response to ozone in mice deficient in TLR4 |
|
