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Sample GSM52185

Query DataSets for GSM52185

Status

Public on Jul 21, 2005

Title

HEK293_HNF4a2_C106R_1+

Sample type

RNA

Source name

HEK293 cell line #1 with induced HNF4a2 mutant C106R

Organism

Homo sapiens

Extracted molecule

total RNA

Description

Analysis of gene expression in human embryonic kidney cells HEK293 overexpressing the transcription factors HNF4a2 or mutant forms HNF4a2 C106R and HNF4a2 R154X, which were introduced by FRT/FLP recombination. The analysis was performed 24 hours following 1µg/ml tetracycline treatment to induce the expression of the genes. Results identify HNF4a regulated genes in kidney cells being several of these genes deregulated in renal cell carcinoma.
Keywords = HEK293
Keywords = embryonic kidney cells
Keywords = HNF4a2
Keywords = tetracycline
Keywords = FRT

Submission date

May 24, 2005

Last update date

Oct 28, 2005

Contact name

Ludger Klein-Hitpass

E-mail(s)

[email protected]

Phone

+49 201 723 85552

Organization name

Institut fuer Zellbiologie

Department

Universitaetsklinikum

Lab

BioChip Lab

Street address

Virchowstr. 173

City

Essen

ZIP/Postal code

D-45122

Country

Germany

Platform ID

GPL96

Series (1)

GSE2700

HEK293 cells overexpressing wild type HNF4a2 or mutant forms HNF4a2 C106R or HNF4a2 R154X

Data table header descriptions
ID_REF
VALUE	'Signal, a measure of the abundance of the transcript
ABS_CALL	the call in an absolute analysis that indicates whether a transcript is reliably detected (Present, P) or not detected (Absent, A)
DETECTION P-VALUE	p-value that indicates the significance level of the detection (ABS_CALL) call

Data table
ID_REF	VALUE	ABS_CALL	DETECTION P-VALUE
AFFX-BioB-5_at	458.7	P	0.002275
AFFX-BioB-M_at	868.7	P	0.000446
AFFX-BioB-3_at	582.2	P	0.003212
AFFX-BioC-5_at	1503	P	0.00006
AFFX-BioC-3_at	1244	P	0.000081
AFFX-BioDn-5_at	951.5	P	0.000446
AFFX-BioDn-3_at	7098.5	P	0.000052
AFFX-CreX-5_at	14378.6	P	0.000044
AFFX-CreX-3_at	26602.9	P	0.000044
AFFX-DapX-5_at	159.1	P	0.020022
AFFX-DapX-M_at	379.8	P	0.028457
AFFX-DapX-3_at	404.6	P	0.001796
AFFX-LysX-5_at	998.4	P	0.00007
AFFX-LysX-M_at	1527.6	P	0.000044
AFFX-LysX-3_at	2492.5	P	0.00007
AFFX-PheX-5_at	4926.4	P	0.000044
AFFX-PheX-M_at	7516.6	P	0.000044
AFFX-PheX-3_at	10283.3	P	0.000044
AFFX-ThrX-5_at	928.1	P	0.000052
AFFX-ThrX-M_at	1021	P	0.000052

Total number of rows: 22283

Table truncated, full table size 600 Kbytes.

Supplementary data files not provided