|
| Status |
Public on Apr 29, 2021 |
| Title |
Ewing sarcoma sample EW724 |
| Sample type |
RNA |
| |
|
| Source name |
Ewing sarcoma sample EW724
|
| Organism |
Homo sapiens |
| Characteristics |
gender: Female
age: 14
tumor type: primary tumor
|
| Treatment protocol |
Tumor sample were stored at -80°C until extraction procedure
|
| Growth protocol |
Tumor sample were fozen in liquid nitrogen or on dry ice
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100 ng total RNA usin 3'IVT Express Kit.
|
| |
|
| Hybridization protocol |
As according to the manufacturer's instructions.
|
| Scan protocol |
GCOS 1.4
|
| Data processing |
All data were normalized using the gcrma package version 2.22.0 in R 2.12 environment with quantile normalization and hgu133plus2hsentrezg Brainarray annotation version 14 (http://brainarray.mbni.med.umich.edu/Brainarray/).
|
| |
|
| Submission date |
Apr 19, 2021 |
| Last update date |
Apr 29, 2021 |
| Contact name |
Didier Surdez |
| Organization name |
Institut Curie
|
| Street address |
26 rue d'ULM
|
| City |
Paris |
| ZIP/Postal code |
75005 |
| Country |
France |
| |
|
| Platform ID |
GPL16311 |
| Series (2) |
| GSE133228 |
STAG2 promotes CTCF-anchored loop extrusion and cis-promoter and -enhancer interactions |
| GSE142162 |
Expression profiling of Ewing sarcoma samples |
|
