|
Status |
Public on Oct 01, 2010 |
Title |
CLL cells PB patient #13 replicate1 |
Sample type |
RNA |
|
|
Source name |
PB derived CLL cells
|
Organism |
Homo sapiens |
Characteristics |
cell type: CLL origin: Peripheral blood patient: patient #13
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted using Qiagen RNA extraction kit
|
Label |
biotin
|
Label protocol |
Biotin labeled with Affymetrix's IVT labeling kits (Affymetrix, Santa Clara, CA).
|
|
|
Hybridization protocol |
Fragmented RNA was assessed for relative length on Agilent 2100 bioanalyzer and hybridized to Affymetrix HG-U133 Plus 2.0 chips for 16 hours.
|
Scan protocol |
Affymetrix GeneChip scanner
|
Description |
primary CLL cells
|
Data processing |
Intensities were pre-processed with MAS5.0, and further processed with variance stablization transformation
|
|
|
Submission date |
Mar 23, 2010 |
Last update date |
Nov 14, 2018 |
Contact name |
Adrian Wiestner |
E-mail(s) |
[email protected]
|
Phone |
301-594-6855
|
Organization name |
NHLBI/NIH
|
Department |
Hematology Branch
|
Street address |
10 Center Dr
|
City |
Bethesda |
State/province |
MD |
ZIP/Postal code |
20892 |
Country |
USA |
|
|
Platform ID |
GPL570 |
Series (1) |
GSE21029 |
The lymph node microenvironment promotes B-cell receptor signaling, NF-κB activation, and tumor proliferation in chronic lymphocytic leukemia (CLL) |
|
Relations |
Reanalyzed by |
GSE122511 |