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Sample GSM529754 Query DataSets for GSM529754
Status Public on Apr 02, 2010
Title Quadriceps muscle at T0, control patient 2
Sample type RNA
 
Source name Quadriceps muscle, control patient, T0
Organism Homo sapiens
Characteristics tissue type: Quadriceps muscle
patient group: total knee arthroplasty control patient
time point: T0
Treatment protocol Patients in the treatment group received ischaemic preconditioning prior to total knee replacement surgery. Ischaemic preconditioning consisted of three five-minute periods of tourniquet insulflation on the lower operative limb, interrupted by five-minute periods of reperfusion.
Extracted molecule total RNA
Extraction protocol TriReagent extraction of RNA was carried out according to the manufacturer's instructions.
Label Biotin
Label protocol Biotinylated cRNA was prepared according to the standard Affymetrix protocol for 6ug of total RNA (Expression Analysis Technical Manual, 2001, Affymetrix.
 
Hybridization protocol Following fragmentation, 10ug of cRNA were hybridized for 16 hours at 45oC on the Affymetrix U113 Plus 2.0 array. The array was then washed and stained on the GeneChip Fluidics Station 450.
Scan protocol The array was scanned using the GeneChip Scanner 3000.
Description Gene expression data from quadriceps muscle biopsy of control patient
Data processing The raw data from the scanner were analysed using the Rosetta Error Model (Rosetta Resolver 7.0) to generate processed intensity values. The following gene filtering was applied to the data: intensity p-value filter, background filter, fold change filter and signature p-value filter.
 
Submission date Apr 01, 2010
Last update date Apr 01, 2010
Contact name Kevin Mulhall
Organization name University College Dublin
Street address Belfield
City Dublin
ZIP/Postal code n/a
Country Ireland
 
Platform ID GPL570
Series (1)
GSE21164 Expression data from total knee arthroplasty patients

Data table header descriptions
ID_REF
VALUE Processed signal intensity

Data table
ID_REF VALUE
1570571_at 28.49406
1570572_at 1.36704
1570574_at 2.10546
1570575_at -0.95462
1570578_at 0.85025
1570579_at 10.68451
1570584_at 0.10729
1570585_at 1.77195
1570587_at 2.86233
1570588_at 28.64354
1570592_a_at 1.12783
1570593_at -3.90209
1570594_at 0.08764
1570596_at 0.76108
1570597_at -1.28869
1570600_at 1.32504
1570601_at 0.51022
1570607_at -3.17391
1570618_at 1.52536
1570621_at -0.27515

Total number of rows: 54675

Table truncated, full table size 1045 Kbytes.




Supplementary file Size Download File type/resource
GSM529754.CEL.gz 7.3 Mb (ftp)(http) CEL
Processed data included within Sample table

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