|
| Status |
Public on Apr 02, 2010 |
| Title |
Quadriceps muscle at T1, treatment patient 3 |
| Sample type |
RNA |
| |
|
| Source name |
Quadriceps muscle, treatment patient, T1
|
| Organism |
Homo sapiens |
| Characteristics |
tissue type: Quadriceps muscle
patient group: total knee arthroplasty treatment patient
time point: T1
|
| Treatment protocol |
Patients in the treatment group received ischaemic preconditioning prior to total knee replacement surgery. Ischaemic preconditioning consisted of three five-minute periods of tourniquet insulflation on the lower operative limb, interrupted by five-minute periods of reperfusion.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
TriReagent extraction of RNA was carried out according to the manufacturer's instructions.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA was prepared according to the standard Affymetrix protocol for 6ug of total RNA (Expression Analysis Technical Manual, 2001, Affymetrix.
|
| |
|
| Hybridization protocol |
Following fragmentation, 10ug of cRNA were hybridized for 16 hours at 45oC on the Affymetrix U113 Plus 2.0 array. The array was then washed and stained on the GeneChip Fluidics Station 450.
|
| Scan protocol |
The array was scanned using the GeneChip Scanner 3000.
|
| Description |
Gene expression data from quadriceps muscle biopsy of treatment patient
|
| Data processing |
The raw data from the scanner were analysed using the Rosetta Error Model (Rosetta Resolver 7.0) to generate processed intensity values. The following gene filtering was applied to the data: intensity p-value filter, background filter, fold change filter and signature p-value filter.
|
| |
|
| Submission date |
Apr 01, 2010 |
| Last update date |
Apr 01, 2010 |
| Contact name |
Kevin Mulhall |
| Organization name |
University College Dublin
|
| Street address |
Belfield
|
| City |
Dublin |
| ZIP/Postal code |
n/a |
| Country |
Ireland |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE21164 |
Expression data from total knee arthroplasty patients |
|
