|
| Status |
Public on Oct 16, 2011 |
| Title |
fertile_donor3 |
| Sample type |
SRA |
| |
|
| Source name |
spermatozoa
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Spermatozoa
|
| Treatment protocol |
semen samples were frozen upon reception.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
samples were thawed and the sperm cells were separated from contaminants through a 50% Percoll cushion. The sperm cells were then processed for separate extraction of small (<200 b) and large (> 200b) RNA using Qiagen Rneasy kit
|
| |
|
| Library strategy |
ncRNA-Seq |
| Library source |
transcriptomic |
| Library selection |
size fractionation |
| Instrument model |
Illumina Genome Analyzer II |
| |
|
| Data processing |
Alignment: Sequence reads obtained from Bustard (Illumina Genome Analyzer II) were mapped to the human (February, 2009) genome using Novoalign (2.05.33MT). Aligned sequence reads for each of the three samples were separated into two classes. Those reads mapping to a unique region on the genome were classified as Uniquely Mapped (UM). Sequence reads mapping to 2–10 locations were classified as Multiple Mapped (MM).
|
| |
|
| Submission date |
Apr 04, 2010 |
| Last update date |
May 15, 2019 |
| Contact name |
Stephen A Krawetz |
| E-mail(s) |
[email protected]
|
| Phone |
313-577-0765
|
| URL |
http://compbio.med.wayne.edu
|
| Organization name |
Wayne State University
|
| Department |
Obstetrics and Gynecology
|
| Street address |
275 E. Hancock, CS Mott Center
|
| City |
Detroit |
| State/province |
MI |
| ZIP/Postal code |
48201 |
| Country |
USA |
| |
|
| Platform ID |
GPL9115 |
| Series (1) |
| GSE21191 |
Characterization of the small RNA population in Human spermatozoa |
|
| Relations |
| SRA |
SRX019961 |
| BioSample |
SAMN00012324 |
