|
| Status |
Public on Jul 01, 2011 |
| Title |
T1+2 |
| Sample type |
RNA |
| |
|
| Source name |
murine colon epithelium
|
| Organism |
Mus musculus |
| Characteristics |
cell line: IEC-6
tissue: colon epithelium
|
| Treatment protocol |
Mice were fed a diet containing 0.4% UDCA.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA according to the manufacturers instructions.
|
| Label |
biotin
|
| Label protocol |
Biotynylated cRNA were prepared according to standard Affymetrix protocol from 3 µg total RNA
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 µg of cRNA were hybridized hybridized for 16 hours at 45°C to an Affymetrix mouse 430A 2.0 GeneChip array.
|
| Scan protocol |
Probe arrays were scanned at 3-μm resolution using the Affymetrix GeneChip System confocal scanner 3000.
|
| Description |
Gene expression in treated mice
|
| Data processing |
Raw data were analyzed with the Affymetrix GeneChip Operating Software (GCOS) version 1.4.
|
| |
|
| Submission date |
Apr 08, 2010 |
| Last update date |
Jul 01, 2011 |
| Contact name |
christoph Hanski |
| E-mail(s) |
[email protected]
|
| Organization name |
Charité Campus Benjamin Franklin
|
| Street address |
Hindenburgdamm 30
|
| City |
Berlin |
| ZIP/Postal code |
12200 |
| Country |
Germany |
| |
|
| Platform ID |
GPL8321 |
| Series (1) |
| GSE21266 |
Effect of Ursodeoxycholic acid on gene expression in the intestial epithelium |
|
