|
| Status |
Public on Dec 16, 2022 |
| Title |
CD4CM-10 |
| Sample type |
genomic |
| |
|
| Source name |
Human Peripheral Blood CD4+ CD45RO+ CD45RA- CD62L+ CD197+ Central memory T cells from leukoreduction filter
|
| Organism |
Homo sapiens |
| Characteristics |
age: 69
horvath_age: NA
purity: 94.5
ethnicity_wide: NA
ethnic_self: NA
meth_ethnicity: WHITE
weight_kg: NA
height_cm: NA
smoker: NA
bmi: NA
bmi_clas: NA
Sex: M
smp_type: FACS isolated cells
arraytypes: IlluminaHumanMethylationEPIC
array: R02C01
slide: 204372030109
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
genomic DNA was extracted and purified from LBC samples using Qiagen DNeasy Kit according to standard instructions
|
| Label |
Cy5 and Cy3
|
| Label protocol |
Standard Illumina Protocol
|
| |
|
| Hybridization protocol |
bisulphite converted DNA was amplified, fragmented and hybridised to Illumina Infinium Human MethylationEPIC Beadchip using standard Illumina protocol
|
| Scan protocol |
Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
|
| Description |
CD4CM
204372030109_R07C01
|
| Data processing |
BeadStudio software v3.2
matrix processed value definition: SeSAMe Processed Average Beta
matrix signal intensities value definition: Unmethylated and methylated signal intensities
|
| |
|
| Submission date |
May 18, 2021 |
| Last update date |
Dec 18, 2022 |
| Contact name |
Lucas A. Salas |
| E-mail(s) |
[email protected]
|
| Organization name |
Geisel School of Medicine at Dartmouth
|
| Department |
Epidemiology
|
| Lab |
Salas Lab
|
| Street address |
1 Medical Center Dr, DHMC
|
| City |
Lebanon |
| State/province |
NH |
| ZIP/Postal code |
03756 |
| Country |
USA |
| |
|
| Platform ID |
GPL21145 |
| Series (1) |
| GSE174666 |
Comparative analysis of the DNA methylation landscape in CD4, CD8, and B memory lineages |
|
