|
| Status |
Public on May 25, 2021 |
| Title |
ACHN_NC_rep.1 |
| Sample type |
SRA |
| |
|
| Source name |
ACHN
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: ACHN
sirna treatment: negative control
|
| Extracted molecule |
total RNA |
| Extraction protocol |
After transfection for 48 h, total RNA was extracted using RNAiso Plus (Takara, #9108Q) according to the manufacturer’s instructions.
mRNA library construction and single end sequencing were executed by BGI (Wuhan, China).
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
BGISEQ-500 |
| |
|
| Data processing |
RNA-Seq data were aligned and quantified using RSEM as well as STAR against the GRCh37 human reference genome and annotation (GTF format) downloaded from GENCODE official website. Gene counts were then used for downstream analysis.
Genome_build: hg19
|
| |
|
| Submission date |
May 24, 2021 |
| Last update date |
May 25, 2021 |
| Contact name |
yuntan qiu |
| E-mail(s) |
[email protected]
|
| Phone |
+86-13016773722
|
| Organization name |
Sun Yat-Sen Memorial Hospital
|
| Department |
Research Center of Medicine
|
| Street address |
107 Yanjiang West Road, Guangzhou City, Guangdong Province
|
| City |
guangzhou |
| State/province |
guangdong |
| ZIP/Postal code |
510000 |
| Country |
China |
| |
|
| Platform ID |
GPL23227 |
| Series (2) |
| GSE175404 |
The RNA binding protein MEX3A controls G1/S transition via regulating RB/E2F pathway in clear cell renal cell carcinoma [RNA-Seq] |
| GSE175409 |
The RNA binding protein MEX3A controls G1/S transition via regulating RB/E2F pathway in clear cell renal cell carcinoma |
|
| Relations |
| BioSample |
SAMN19316650 |
| SRA |
SRX10971134 |
