|
| Status |
Public on Feb 08, 2011 |
| Title |
HCC5-N |
| Sample type |
RNA |
| |
|
| Source name |
Hepatocellular Carcinoma in Milan Criteria Cases with Mild Liver Cirrhosis
|
| Organism |
Homo sapiens |
| Characteristics |
tissue type: Non-tumor
sample id: HCC5
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Tumor and non-tumor tissues were obtained and frozen in liquid nitrogen immediately after hepatic resection, and stored in liquid nitrogen until RNA extraction. Total RNA samples were extracted by a phenol-chloroform RNA extraction method.
|
| Label |
Hy5
|
| Label protocol |
For each patient, 500 ng of total RNA derived from tumor and non-tumor samples were labeled using miRCURY LNA™ microRNA Power Labeling Kits Hy5
|
| |
|
| Hybridization protocol |
The labeled samples were individually hybridized onto DNA chip surface, and incubated at 42 ℃ for 16 hours.
|
| Scan protocol |
Washed and dried DNA chip in an ozone-removed environment was scanned using a ProScanArray™ microarray scanner. The obtained microarray images were analyzed using the Genepix Pro™ 4.0 software.
|
| Description |
Total miRNA was spotted on 934 features. We used only human miRNA in our analysis. Therefore, a total of 68 features were already eliminated from the all features on this array.
|
| Data processing |
The median values of the foreground signal minus the local background were represented as feature intensities. All data obtained from microarray experiments were normalized by a quantile normalization method, and filtered.
|
| |
|
| Submission date |
Apr 16, 2010 |
| Last update date |
Feb 09, 2011 |
| Contact name |
Akira Myomoto |
| Organization name |
Kyoto Univ.
|
| Street address |
Yoshida-Shimoadachi
|
| City |
Kyoto |
| ZIP/Postal code |
6068501 |
| Country |
Japan |
| |
|
| Platform ID |
GPL10312 |
| Series (1) |
| GSE21362 |
MicroRNA Profile Predicts Recurrence of Hepatocellular Carcinoma in Milan Criteria Cases with Mild Liver Cirrhosis. |
|
