|
| Status |
Public on Apr 21, 2010 |
| Title |
ovary_Low prolificacy level_animal2 |
| Sample type |
RNA |
| |
|
| Source name |
ovary sample at 30 days after fertilization, low
|
| Organism |
Sus scrofa |
| Characteristics |
breed: Iberian x Meishan F2 cross
tissue: ovary
prolificacy: lower EBV (LOW prolificacy)
|
| Treatment protocol |
Ovary samples were mantained at -80ºC
|
| Growth protocol |
Sows were slaughtered after 30 days of pregnancy
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RiboPure RNA isolation kit
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol
|
| |
|
| Hybridization protocol |
standard Affymetrix hybridization protocol
|
| Scan protocol |
standard Affymetrix scanning protocol using GCOS
|
| Description |
OVARY L-2
|
| Data processing |
Expression data were generated with GeneChip Operating Software (GCOS, Affymetrix) and GCRMA normalization was carried out with BRB-Array Tools (v.3.6.0), by filtering out those probes with more than 50% of missing values.
|
| |
|
| Submission date |
Apr 19, 2010 |
| Last update date |
Apr 21, 2010 |
| Contact name |
Amanda Fernández Rodríguez |
| E-mail(s) |
[email protected]
|
| Phone |
+34913473792
|
| Organization name |
INIA
|
| Department |
Mejora Genética Animal
|
| Street address |
Ctra Coruña km 7.5
|
| City |
Madrid |
| State/province |
Madrid |
| ZIP/Postal code |
28040 |
| Country |
Spain |
| |
|
| Platform ID |
GPL3533 |
| Series (1) |
| GSE21383 |
Expression data from porcine ovary tissue of sows from two prolificacy levels |
|
