|
| Status |
Public on Sep 08, 2021 |
| Title |
NT_10d_1 |
| Sample type |
SRA |
| |
|
| Source name |
oocyte
|
| Organism |
Mus musculus |
| Characteristics |
strain: Kunming
tissue: ovary
treatment: derived from follicles grown in vitro with NT-4 for 10 days
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Each sample containing 6 oocytes were collected in lysate buffer. Whole oocyte total RNA was amplified using the SMARTSeq2 protocol. Paired-end sequencing was performed with an Illumina Novaseq™ 6000.
RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Data processing |
FastQC filtering
HISAT2 aligment
edgeR transcript abundance estimation
Genome_build: mm10
Supplementary_files_format_and_content: tab-delimited text files include raw count values for each Sample ...
|
| |
|
| Submission date |
Jun 09, 2021 |
| Last update date |
Sep 08, 2021 |
| Contact name |
Ying-chun Guo |
| E-mail(s) |
[email protected]
|
| Organization name |
Sixth Affiliated Hospital of Sun Yat-sen University
|
| Street address |
NO.17, Shougouling Rd.
|
| City |
Guangzhou |
| ZIP/Postal code |
510275 |
| Country |
China |
| |
|
| Platform ID |
GPL24247 |
| Series (2) |
| GSE176516 |
Single-cell transcriptome and cell-specific network analysis reveal the reparative effect of NT-4 supplementation in preantral follicles grown in vitro [oocyte_RNA-seq] |
| GSE177031 |
Single-cell transcriptome and cell-specific network analysis reveal the reparative effect of NT-4 supplementation in preantral follicles grown in vitro |
|
| Relations |
| BioSample |
SAMN19651073 |
| SRA |
SRX11105237 |
