|
| Status |
Public on Jun 18, 2021 |
| Title |
Young SIRT6 heterozygous mouse brain, 21 days old, biological replicate 3 |
| Sample type |
RNA |
| |
|
| Source name |
young het
|
| Organism |
Mus musculus |
| Characteristics |
age: 21 days
genotype: het
tissue: brain
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol reagent RNA extraction followed by additional purification using RNeasy from Qiagen
|
| Label |
biotin
|
| Label protocol |
standard Affymetrix protocol
|
| |
|
| Hybridization protocol |
standard Affymetrix protocol
|
| Scan protocol |
standard Affymetrix protocol
|
| Description |
brain sample of 21-day-old heterozygous (+/-) mouse. In this animal model, SIRT6WT (+/+) and SIRT6het (+/-) present no phenotypical differences.
|
| Data processing |
Data was processed using Affymetrix Power Tools, APT version 1.10.1, with the following parameters: #%affymetrix-algorithm-param-apt-opt-analysis-spec=rma-bg,quant-norm.sketch=-1.bioc=true.lowprecision=false.usepm=true.target=0.doavg=false.subsetmd5=6939e6d4c979d793c87e21aab959a6b4,pm-only,med-polish.FixFeatureEffect=false.UseInputModel=false.FitFeatureResponse=true.expon=false.attenuate=true.l=0.005.h=-1,expr.genotype=false.strand=false.allele-a=false
|
| |
|
| Submission date |
Jun 17, 2021 |
| Last update date |
Jun 20, 2021 |
| Contact name |
Debra Toiber |
| E-mail(s) |
[email protected], [email protected]
|
| Phone |
0584449189
|
| Organization name |
Ben Gurion University of the Negev
|
| Street address |
Ben Gurion Road #1, Building 40, room 205
|
| City |
Beer Sheva |
| State/province |
Israel |
| ZIP/Postal code |
8410501 |
| Country |
Israel |
| |
|
| Platform ID |
GPL6193 |
| Series (1) |
| GSE178432 |
Expression data from SIRT6 knockout, young WT and old WT mice brains |
|
