|
| Status |
Public on Nov 03, 2010 |
| Title |
WT E2 2h |
| Sample type |
RNA |
| |
|
| Source name |
breast epithelial cell, adherent, E2, 2h
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: MCF-7
tissue origination: mammary gland, breast
cell type: epithelial
disease: adenocarcinoma
age: 69
gender: female
ethnicity: Caucasian
tamoxifen phenotype: sensitive
treatment: E2
time: 2h
|
| Biomaterial provider |
American Type Culture Collection (ATCC)
|
| Treatment protocol |
Following the incubation in serum-free medium, the ligand was added and incubated for the indicated time.
|
| Growth protocol |
Prior to the growth factor (GF) treatment, cells were serum starved for 16-18 hours with serum-free RPMI medium and incubated at 37C/5% CO2. The medium was again changed 2 hr prior to the GF treatment.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using TRIzol reagent (Invitrogen) and then purified using the QIAGEN Rneasy kit.
|
| Label |
biotin
|
| Label protocol |
Standard Affymetrix Protocols (Affymetrix GeneChip Expression Analysis Technical Manual) was used. Complete protocol can be found on the manufacturer's web site (http://www.affymetrix.com/support/technical/manual/expression_manual.affx) (section2, chapter1-p13-18, & 2.1.p32-33,p36-40).
|
| |
|
| Hybridization protocol |
Standard Affymetrix Protocols (Affymetrix GeneChip Expression Analysis Technical Manual) was used. Complete protocol can be found on the manufacturer's web site (http://www.affymetrix.com/support/technical/manual/expression_manual.affx) (section2, 2.2.p3-8 & 2.3.p3-14).
|
| Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000 with default parameters.
|
| Description |
Gene expression in E2-stimulated WT cells.
wt4.E2.02h
|
| Data processing |
The data were preprocessed with RMA. Signal intensity was calculated for RefSeq mRNA by using a CustomCDF (HGU133Plus2_Hs_REFSEQ ver.11).
|
| |
|
| Submission date |
Apr 30, 2010 |
| Last update date |
Nov 03, 2010 |
| Contact name |
Mariko Okada |
| E-mail(s) |
[email protected]
|
| Organization name |
RIKEN RCAI
|
| Lab |
Laboratory for Cellular Systems Modeling
|
| Street address |
W518, 1-7-22, Suehiro-cho, Tsurumi-ku
|
| City |
Yokohama |
| ZIP/Postal code |
230-0045 |
| Country |
Japan |
| |
|
| Platform ID |
GPL10371 |
| Series (1) |
| GSE21618 |
Expression profiling of ligand-stimulated wild type and tamoxifen-resistant MCF-7 |
|
