|
| Status |
Public on Sep 15, 2010 |
| Title |
Free-living mycelium 4°C 1 |
| Sample type |
RNA |
| |
|
| Source name |
free-living mycelium, 4°C
|
| Organism |
Tuber melanosporum |
| Characteristics |
strain: Mel28
tissue: free-living mycelium
treatment: 4°C
|
| Biomaterial provider |
Institut National de la Recherche Agronomique (INRA Nancy, France)
|
| Growth protocol |
The fungus was grown in flasks containing 50 ml of malt 1% liquid medium. The cultures were maintained in a dark room at 25°C. After four weeks, the flasks were moved to 4°C for 1 week.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA extraction was carried out using the RNeasy Plant Mini Kit (Qiagen). Total RNA preparations were amplified using the SMART PCR cDNA Synthesis Kit (Clontech) according to the manufacturer’s instructions
|
| Label |
Cy3
|
| Label protocol |
Labelling by NimblGen Company
|
| |
|
| Hybridization protocol |
Hybridization by NimblGen Company
|
| Scan protocol |
Scanning by NimblGen Company
|
| Description |
Procedure for spot detection was performed by NimbleGen Company
|
| Data processing |
Expression values reflect across-array quantile normalization and gene calls generated using the Robust Multichip Average (RMA).
For information on RMA, see Biostatistics 2003 Apr; 4(2): 249-264
|
| |
|
| Submission date |
May 03, 2010 |
| Last update date |
Jun 25, 2010 |
| Contact name |
Annegret Kohler |
| E-mail(s) |
[email protected]
|
| Phone |
+33 (0)383 394072
|
| Organization name |
INRAE
|
| Department |
UMR 1136
|
| Lab |
Interactions Arbres/Micro-organismes
|
| Street address |
Centre INRAE Grand Est Nancy
|
| City |
Champenoux |
| ZIP/Postal code |
54280 |
| Country |
France |
| |
|
| Platform ID |
GPL8982 |
| Series (1) |
| GSE21626 |
The black truffle of Perigord responds to a temperature shift with an extensive reprogramming of its transcriptional activity |
|
