|
| Status |
Public on Jun 23, 2011 |
| Title |
MEL_PU.1_A1 |
| Sample type |
SRA |
| |
|
| Source name |
Murine erythroleukemia (MEL) cells stably transfected with Gata-1 fused to ER
|
| Organism |
Mus musculus |
| Characteristics |
clone #: 4
chip antibody: anti-PU.1 (T-21)
antibody vendor: Santa Cruz
antibody catalog #: sc-352
antibody lot #: K2508
|
| Growth protocol |
MEL cells were grown in DMEM supplemented with 10% FBS (Gemini), 2mM L-glutamine, and 5mg/ml puromycin. ES-EPs were cultured in StemPro34 media (Invitrogen) supplmented with 2u/ml Epogen (Amgen), 40ng/ml hIGF-1 (Sigma), 10-6M dexamethosone (Sigma), 100ng/ml murine SCF (R&D Systems/Invitrogen), and 0.1% -mercaptoethanol (Gibco). ES-EP were maintained between 1-6x106 cells/ml by adding fresh media daily.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Libraries were prepared as recommended by Illumina.
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina Genome Analyzer II |
| |
|
| Description |
Chromatin immunoprecipitation was performed using an antibody against PU.1
|
| Data processing |
Peak detection to mm9 was done using cisGenome and spp software.
|
| |
|
| Submission date |
May 21, 2010 |
| Last update date |
May 15, 2019 |
| Contact name |
Sandeep Nagaraj Wontakal |
| Organization name |
Albert Einstein College of Medicine
|
| Department |
Cell Biology
|
| Lab |
Dr. Art Skoultchi
|
| Street address |
1300 Morris Park Ave Chanin 402
|
| City |
Bronx |
| State/province |
NY |
| ZIP/Postal code |
10461 |
| Country |
USA |
| |
|
| Platform ID |
GPL9250 |
| Series (2) |
| GSE21950 |
Genome-wide binding pattern of PU.1 in normal erythroid progenitors and erythroleukemia cells |
| GSE21953 |
PU.1 in normal erythroid progenitors and erythroleukemia cells |
|
| Relations |
| SRA |
SRX021429 |
| BioSample |
SAMN00014129 |
