|
| Status |
Public on Oct 13, 2021 |
| Title |
PUT_81_Case |
| Sample type |
RNA |
| |
|
| Source name |
Human postmortem putamen
|
| Organism |
Homo sapiens |
| Characteristics |
aud status: Case
alcoholdailyuse(gram/day): 80
Sex: Male
batch: 2
rin: 5.1
brainweight: 1400
brainph: 6.29
pmi: 29
leftrightbrain: Right
causeofdeathcategory: HepaticBloodLoss
smoking: Former
liverclass: Steatosis
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with the Trizol reagent using the QIAGEN RNeasy mini kit and followed by clean-up and DNase I treatment in accordance with the prescribed protocol provided with the kit. Quality control was performed with the Agilent Bioanalyzer System.
|
| Label |
biotin
|
| Label protocol |
More than 100 ng of total RNA was used in the Affymetrix miRNA4.0 array assay. RNAs were labeled using the FlashTag Biotin Labeling kit and hybridized to the array.
|
| |
|
| Hybridization protocol |
A “rotisserie” oven was used to carry out the array hybridization at 48°C and 60 rpm for 16 to 18 hours.
|
| Scan protocol |
GeneChips were scanned using GeneChip Scanner 3000 with Autoloader.
|
| Description |
miRNA transcriptome data
|
| Data processing |
Small noncoding RNA CEL files were processed using the Affymetrix Expression Console (EC) software (v1.4.1)
|
| |
|
| Submission date |
Jul 23, 2021 |
| Last update date |
Oct 13, 2021 |
| Contact name |
Huiping Zhang |
| E-mail(s) |
[email protected]
|
| Phone |
6173583689
|
| Organization name |
Boston University School of Medicine
|
| Department |
Psychiatry
|
| Street address |
72 East Concord Street
|
| City |
Boston |
| State/province |
Massachusetts |
| ZIP/Postal code |
02118 |
| Country |
USA |
| |
|
| Platform ID |
GPL21572 |
| Series (1) |
| GSE180700 |
Profiling miRNA transcriptomic changes using Affymetrix miRNA4.0 in 6 regions of postmortem brains of subjects with alcohol use disorder |
|
