|
| Status |
Public on Dec 21, 2021 |
| Title |
Isotype 1 |
| Sample type |
SRA |
| |
|
| Source name |
CD45+ cells sorted from B16 tumor
|
| Organism |
Mus musculus |
| Characteristics |
strain: B16-F10 tumor bearing mice
treatment: Isotype antibody
|
| Treatment protocol |
Mice were treated with isotype or triple combinatorial therapy at days 10, 13 and 16 post-tumor implantation
|
| Growth protocol |
5x10^5 B16-F10 tumor cells were implanted s.c into mice and tumor resected 21 days post s.c. implantation.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
TILs were isolated by digesting tumor fragments using Mylteni mouse tumor disassociation kit with enzyme R at 1:10 per manufacturer's guidelines. 1 to 5 × 106 cells were stained using fluorescently labeled mouse CD45 antibody (clone 30F11) and Fixable Viability Dye eFluor 506 (Thermo Fisher Scientific; Waltham, MA) to sort for live CD45+ and CD45− cells using the FACSAria II cell sorter.
RNA sequencing by standard protocol
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Description |
CD45+ cells sorted from B16 tumor after treatment with isotype antibody
|
| Data processing |
Data were processed using the Cell Ranger v2.1.1 (10x Genomics) to generate count-level cell by gene matrix for further analysis.
Genome_build: mm10
|
| |
|
| Submission date |
Jul 29, 2021 |
| Last update date |
Dec 21, 2021 |
| Contact name |
Wenjing Yanf |
| E-mail(s) |
[email protected]
|
| Organization name |
Pfizer
|
| Department |
Oncology
|
| Street address |
10770 Science Center Drive
|
| City |
San Diego |
| State/province |
CA |
| ZIP/Postal code |
92121 |
| Country |
USA |
| |
|
| Platform ID |
GPL24247 |
| Series (2) |
| GSE181142 |
Combinatorial Immunotherapy Induces Tumor Infiltrating CD8+ T Cells with Distinct Functional, Migratory, and Stem-Like Properties I |
| GSE181152 |
Combinatorial Immunotherapy Induces Tumor Infiltrating CD8+ T Cells with Distinct Functional, Migratory, and Stem-Like Properties |
|
| Relations |
| BioSample |
SAMN20491691 |
| SRA |
SRX11604612 |
