|
| Status |
Public on Sep 23, 2021 |
| Title |
In labor myometrium rep12 |
| Sample type |
SRA |
| |
|
| Source name |
Myometrium
|
| Organism |
Homo sapiens |
| Characteristics |
ga at delivery (weeks+days): 38+4
labor durations (hours): 18
cervical dilatation（cm）: 3
maternal age (years): 29
fetal weight（g）: 2900
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Myometrium were removed, flash frozen on dry ice, and RNA was harvested using Trizol reagent.
RNA libraries were prepared for sequencing using standard Illumina protocols. NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) was used for the construction of sequencing libraries.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
BGISEQ-500 |
| |
|
| Data processing |
fastq files were trimmed with trimmomatic
Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence, then mapped to hg38 whole genome using hisat2.
Genome_build: hg38
Supplementary_files_format_and_content: tab-delimited text files include FPKM values for each Sample.
|
| |
|
| Submission date |
Aug 02, 2021 |
| Last update date |
Sep 23, 2021 |
| Contact name |
Kaiyuan Ji |
| E-mail(s) |
[email protected]
|
| Organization name |
Guangzhou Medical University
|
| Department |
Guangzhou Women and Children’s Medical Center
|
| Lab |
Department of Obstetrics and Gynecology
|
| Street address |
No.9 Jinsui Road, Guangzhou, China
|
| City |
Guangzhou |
| ZIP/Postal code |
510623 |
| Country |
China |
| |
|
| Platform ID |
GPL23227 |
| Series (1) |
| GSE181348 |
Transcriptomes of human myometrium in labor reveals the increased molecular associated with inflammation under hypoxia stress |
|
| Relations |
| BioSample |
SAMN20523446 |
| SRA |
SRX11632584 |
