|
| Status |
Public on Jun 07, 2011 |
| Title |
ALI-D14, donor 2 |
| Sample type |
RNA |
| |
|
| Source name |
Human nasal epithelial cells
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: human nasal mucosa
differentiation step: Onset of mucociliary differentiation
|
| Treatment protocol |
At confluency (day 7 after seeding), cultures cells were switched in air-liquid interface (ALI) in the differentiating medium.
|
| Growth protocol |
Dissociated cells from nasal polyps were either seed on collagen-type IV coated permeable supports for differentiation.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
Cy3
|
| Label protocol |
The microRNAs were labled using the miRNA Complete Labeling and Hyb Kit. Details were that 100 ng total RNA was treated with phosphatase at 37 ℃for 30min. The dephosphorylated RNA was ligated and labeled using the ligation master mix following the kit instructions
|
| |
|
| Hybridization protocol |
Cy3-labeled RNA were hybridized following manufacturer's instructions (MiRNA complete labeling and hybridization kit, Agilent Technologies) 20 hours at 55°C in a rotating Agilent hybridization oven. After hybridization, microarrays were washed 1 minute at room temperature with GE Wash Buffer 1 (Agilent) and 1 minute with 37°C GE Wash buffer 2 (Agilent), then dried immediately by brief centrifugation.
|
| Scan protocol |
Scanned on an Agilent G2565AA Scanner.
|
| Description |
MicroRNA expression data
|
| Data processing |
GeneView files were generated using Agilent's Feature Extraction software version 9.5.3.1
|
| |
|
| Submission date |
Jun 04, 2010 |
| Last update date |
Jun 07, 2011 |
| Contact name |
Kevin Lebrigand |
| Organization name |
IPMC/CNRS
|
| Lab |
Functional Genomics Platform of Nice-Sophia-Antipolis, France.
|
| Street address |
660 route des lucioles
|
| City |
Valbonne - Sophia-Antipolis |
| ZIP/Postal code |
06560 |
| Country |
France |
| |
|
| Platform ID |
GPL8227 |
| Series (2) |
| GSE22141 |
MicroRNA signature during the time course of regeneration of the human airway mucociliary epithelium |
| GSE22147 |
microRNAs-449 control vertebrate multi-ciliogenesis by repressing Notch signalling |
|
