|
| Status |
Public on Dec 13, 2021 |
| Title |
AE104 with 50 µM EDTA replicate 3 |
| Sample type |
RNA |
| |
|
| Source name |
AE104 with 50 µM EDTA
|
| Organism |
Cupriavidus metallidurans |
| Characteristics |
strain background: AE104
treatment: 50 µM EDTA
|
| Treatment protocol |
100 µM ZnCl2 or 50 µM EDTA was added at a cell turbitity of 100 Klett for 10 min
|
| Growth protocol |
C. metallidurans strains were grown in Mineral salts medium + 0.2 % Na-gluconate to a cell turbidity of 100 Klett, 100 µM Zn or 50 µM EDTA was added and after 10 min cells were harvest by centrifugation for 1' at RT in 2.0 ml tubes.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy Plus Kit (Qiagen) was used according to the manufacturers manual including on-column DNase digestion. PCR were done to check for DNA contaminations, only RNA was used that did not generate products in the PCR reaction. RNA quality was checked on formamide gels and measured as RIN on Agilent 2100 Bioanalyzer (Agilent Technologies, Waldbronn, Germany).
|
| Label |
Cy3
|
| Label protocol |
Labeling was done by the service provider IMGM Laboratories GmbH (Martinsried)
|
| |
|
| Hybridization protocol |
Hybridisation was done by the service provider IMGM Laboratories GmbH (Martinsried)
|
| Scan protocol |
Agilent DNA microarray Scanner were used by the service provider IMGM Laboratories GmbH (Martinsried)
|
| Data processing |
The mean intensity of the pixels of the surrounding area was subtracted from the mean density of the pixels of the spots to give the signal strength
|
| |
|
| Submission date |
Aug 10, 2021 |
| Last update date |
Dec 13, 2021 |
| Contact name |
Cornelia Große |
| E-mail(s) |
[email protected]
|
| Organization name |
Martin-Luther-University Halle-Wittenberg
|
| Department |
Faculty of Biology/Microbiology
|
| Street address |
Kurt-Mothes-Str.3
|
| City |
Halle/Saale |
| ZIP/Postal code |
06120 |
| Country |
Germany |
| |
|
| Platform ID |
GPL16115 |
| Series (1) |
| GSE181790 |
Response of efflux system deletion mutants of Cupriavidus metallidurans to Zn and EDTA |
|
