|
| Status |
Public on Oct 13, 2021 |
| Title |
Subject_1_Control [Human postmortem nucleus accumbens] |
| Sample type |
SRA |
| |
|
| Source name |
Human postmortem nucleus accumbens
|
| Organism |
Homo sapiens |
| Characteristics |
aud status: Control
alcoholdailyuse(gram/day): 15
Sex: Male
batch: 2
rin: 5.8
brainweight: 1320
brainph: 6.12
pmi: 29
leftrightbrain: Left
causeofdeathcategory: Cardiac
smoking: Never
liverclass: MildSteatosis
|
| Treatment protocol |
Not applicable
|
| Growth protocol |
Not applicable
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with the Trizol reagent using the QIAGEN RNeasy mini kit and followed by clean-up and DNase I treatment in accordance with the prescribed protocol provided with the kit. Quality control was performed with the Agilent Bioanalyzer System.
The KAPA RNA HyperPrep Kit with RiboErase (KAPA Biosystems, Wilmington, MA, USA) was used to deplete ribosomal RNAs (rRNAs) and construct RNA-seq libraries with 1 ug of total RNAs as the starting material.
Ribosomal RNA depletion RNA-seq
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Description |
mRNA transcriptome data
Sample_97
|
| Data processing |
The bulk RNA-seq processing pipeline Pipeliner [Federico et al. Front Genet 2019; 10, 614] was utilized to quantitate gene expression.
Genome_build: hg19
Supplementary_files_format_and_content: Matrix table with raw gene counts for every gene and every sample
|
| |
|
| Submission date |
Aug 16, 2021 |
| Last update date |
Oct 13, 2021 |
| Contact name |
Huiping Zhang |
| E-mail(s) |
[email protected]
|
| Phone |
6173583689
|
| Organization name |
Boston University School of Medicine
|
| Department |
Psychiatry
|
| Street address |
72 East Concord Street
|
| City |
Boston |
| State/province |
Massachusetts |
| ZIP/Postal code |
02118 |
| Country |
USA |
| |
|
| Platform ID |
GPL16791 |
| Series (2) |
| GSE181982 |
Profiling of transcriptomic changes using seq in postmortem brains of subjects with alcohol use disorder |
| GSE182223 |
Profiling of mRNA transcriptomic changes using RNA-seq in postmortem nucleus accumbens of subjects with alcohol use disorder |
|
| Relations |
| BioSample |
SAMN20818397 |
| SRA |
SRX11787843 |
