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| Status |
Public on Mar 01, 2022 |
| Title |
Hypoosmotic_t180_n3 |
| Sample type |
RNA |
| |
|
| Source name |
perfused rat liver
|
| Organism |
Rattus norvegicus |
| Characteristics |
time point: t180
tissue: liver
strain: wistar
Sex: Male
buffer: hypoosmotic (225 mosm/L)
|
| Treatment protocol |
Rat liver perfusions were conducted as previously described by Sies et al. [https://doi.org/10.1016/s0076-6879(78)52005-3.]
|
| Growth protocol |
Male wistar rats were kept under normal husbandry conditions.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated from homogenized liver samples with the RNeasy Mini Kit (Qiagen), according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cDNA for hybridization to Affymetrix GeneChip arrays was prepared with the Affymetrix GeneChip whole transcript sense target labeling assay, using the standard 1 μg total RNA method according to the manufacturer’s protocols (Affymetrix, 900854).
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| |
|
| Hybridization protocol |
Hybridization were carried out in a SciGene 777 Microarray Oven (50 rpm, 45 °C for 16 hours).Hybridized Affymetrix Arrays were washed by Affymetrix GeneChip Fluidics Station 450.
|
| Scan protocol |
Microarrays scanning and acquisition of signal intensities were performed on the Affymetrix GeneChip Scanner GCS3000 System.
|
| Description |
Gene expression data from hypoosmotic perfused rat liver
|
| Data processing |
Quality control assessment was carried out through the Expression Console software (Affymetrix). Statistical analysis of microarray data was performed by Transcriptome Analysis Console 4.0 (TAC 4.0). CEL files were imported to TAC and the data was preprocessed and normalized using the RMA (Robust Multichip Average) algorithm.
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| |
|
| Submission date |
Sep 05, 2021 |
| Last update date |
Mar 01, 2022 |
| Contact name |
David Schöler |
| Organization name |
University Hospital Düsseldorf
|
| Street address |
Moorenstr. 5
|
| City |
Düsseldorf |
| ZIP/Postal code |
40225 |
| Country |
Germany |
| |
|
| Platform ID |
GPL6247 |
| Series (2) |
| GSE183461 |
Expression data from normo- and hypoosmotic perfused rat liver [180min] |
| GSE183462 |
Expression data from normo- and hypoosmotic perfused rat liver |
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