|
| Status |
Public on Nov 19, 2010 |
| Title |
Wild type EBV-transformed B-cells, biological replicate 1 |
| Sample type |
RNA |
| |
|
| Source name |
Wild type EBV-transformed B-cells
|
| Organism |
Homo sapiens |
| Characteristics |
individual: Healthy donor
gender: female
cell type: B-cell
agent: none
|
| Treatment protocol |
Vitamin D treatment (1,25(OH)2D3 (Biomol, USA))
|
| Growth protocol |
Cells were maintained in RPMI 1640 medium, 37°C in 5% CO2
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy Mini Kit (Qiagen) including DNase treatment
|
| Label |
biotin
|
| Label protocol |
MessageAmp II-Biotin Enhanced 1-cycle protocoll (Ambion)
|
| |
|
| Hybridization protocol |
According to the GeneChip Whole Transcript (WT) Sense Target Labeling Assay
|
| Scan protocol |
According to the Affymetrix expression protocol
|
| Description |
Gene expression data from a B-cell line derived from a healthy donor
wt_minusVitD_1
|
| Data processing |
RMA probeset summary and quantile normalisation (Expression console)
|
| |
|
| Submission date |
Jun 23, 2010 |
| Last update date |
Dec 18, 2013 |
| Contact name |
Johannes Beckers |
| E-mail(s) |
[email protected]
|
| Organization name |
Helmholtz Zentrum Muenchen
|
| Department |
Institute of Experimental Genetics
|
| Street address |
Ingolstaedter Landstr. 1
|
| City |
Neuherberg |
| ZIP/Postal code |
85764 |
| Country |
Germany |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE22523 |
Analysis of vitamin D response element binding protein target genes reveals a role for vitamin D in osteoblast mTOR signaling |
|
