|
| Status |
Public on Jul 01, 2011 |
| Title |
12295-anti-miR-NC (negative control)-2 |
| Sample type |
RNA |
| |
|
| Source name |
HepG2 hepatocellular carcinoma cell line
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HepG2 hepatocellular carcinoma cell line
treatment: anti-miR-NC (negative control)
|
| Treatment protocol |
Cells were transfected with Oligofectamine (Invitrogen, Cat# 12252011) according to manufacturer instructions.
|
| Growth protocol |
Cells were grown in MEM–Eagle (+Earl's BBS), w/NEAA and 10% FBS with additions of sodium bicarbonate and sodium pyruvate.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
EZ-RNA II kit from Biological Industries, Israel, was used for total RNA extraction from cell lines.
|
| Label |
biotin
|
| Label protocol |
DNA is labeled by terminal deoxynucleotidyl transferase (TdT) with the Affymetrix® proprietary DNA Labeling Reagent that is covalently linked to biotin.
|
| |
|
| Hybridization protocol |
Hybridization was done using GeneChip® Hybridization, Wash, and Stain Kit, according to manufacturer instructions. In short hybridization was done in hybridization buffer at 45C over night. The GeneChip® Fluidics Station 450/250 is used to wash and stain the GeneChip® ST Arrays. It is operated using GCOS.
|
| Scan protocol |
The GeneChip® Scanner 3000 7G was used to scan the chips. It is controlled by GeneChip® Operating Software (GCOS).
|
| Description |
12295
|
| Data processing |
Data was analyzed by RMA on the arrays using the expression console software by Affymetrix.
|
| |
|
| Submission date |
Jul 07, 2010 |
| Last update date |
Jul 01, 2011 |
| Contact name |
Einat Sitbon |
| Organization name |
Rosetta Genomics
|
| Street address |
10 Plaut St
|
| City |
Rehovot |
| ZIP/Postal code |
76706 |
| Country |
Israel |
| |
|
| Platform ID |
GPL6244 |
| Series (2) |
| GSE22790 |
Gene expression after treatment with anti-miR-191 or control |
| GSE22910 |
hsa-miR-191, a potential target for Hepatocellular carcinoma therapy |
|
