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Sample GSM563616 Query DataSets for GSM563616
Status Public on Jun 27, 2011
Title Sf301_LB_Sta_rep2
Sample type RNA
 
Source name Shigella flexneri 2a strain 301 wild-type
Organism Shigella flexneri 2a str. 301
Characteristics genotype: Wild-type
phenotype: normal
growth condition: at 37℃ in LB medium, in stationary phase.
Growth protocol S. flexneri 2a strain 301(Sf301) was routinely grown at 37℃ overnight on Luria-Bertani(LB) agar plate containing 0.01% congo red. A single red colony was inoculated into LB medium without antibiotics and grown overnight at 37℃ and 250 r.p.m. For RNA extraction, an overnight culture of bacteria was diluted 1: 50 into 100 ml fresh medium with aeration by rotary shaking (250 r.p.m.)
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using a Promega SV total RNA purification kit according to the manufacturer’s instructions.
Label Cy5
Label protocol The separate labeling reactions were pooled after each respective Cy dye incorporation step and then again divided into aliquots to minimize inconsistencies in probe generation. cDNA was purified with a QIAquick PCR Purification kit (QIAGEN) according to the QIAquick Spin handbook.
 
Hybridization protocol Labeled cDNA samples were individually hybridized to the microarray according to the NimbleGen standard operating procedure. Competitive hybridization was done three times for the sample from one condition.
Scan protocol Microarrays were scanned at a 5μm resolution using a GenePix 4000B scanner (Axon Instruments). Data were extracted using NimbleScan (NimbleGen).
Description This sample is of wild-type Shigella flexneri 2a strain 301 at 37℃ in LB medium, in stationary phase. It is the seond of three wild-type biological replicates used in this experiment, each from separate cultures.
Data processing NMPP(Bioinformatics, 2006,22:2955-2957.)
 
Submission date Jul 07, 2010
Last update date Jun 27, 2011
Contact name Junping Peng
E-mail(s) [email protected]
Organization name Chinese center for disease control and prevention
Lab State key lab for molecular virology and genetic engineering
Street address No 6, Rongjing Eastern Street, BDA
City Beijing
ZIP/Postal code 100176
Country China
 
Platform ID GPL10653
Series (1)
GSE22800 Expression analysis of Shigella flexneri 2a strain 301

Data table header descriptions
ID_REF
VALUE RMA-normalized, averaged gene-level signal intensity

Data table
ID_REF VALUE
AE005674FS000000001 1038.36
AE005674FS000000026 846.37
AE005674FS000000051 877.17
AE005674FS000000076 553.96
AE005674FS000000101 400.05
AE005674FS000000126 663.38
AE005674FS000000151 710.21
AE005674FS000000176 478
AE005674FS000000201 344.75
AE005674FS000000226 277.3
AE005674FS000000251 179.48
AE005674FS000000276 168.36
AE005674FS000000301 3301.31
AE005674FS000000326 3101.1
AE005674FS000000351 2588.25
AE005674FS000000376 780.56
AE005674FS000000401 1016.29
AE005674FS000000426 1682.86
AE005674FS000000451 3830.76
AE005674FS000000476 4284.81

Total number of rows: 389307

Table truncated, full table size 10350 Kbytes.




Supplementary file Size Download File type/resource
GSM563616_38014_635.pair.gz 5.7 Mb (ftp)(http) PAIR
Processed data included within Sample table

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