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Sample GSM5643616 Query DataSets for GSM5643616
Status Public on Oct 22, 2023
Title hAoSMCs treated with vehicle 24h rep1
Sample type RNA
 
Source name hAoSMCs treated with vehicle 24h
Organism Homo sapiens
Characteristics sample type: cell
Treatment protocol Human AoSMCs were seeded in 6-well plates at an appropriate concentration, when the cell density reached 60-70%, hAoVSMCs were starved with serum-free medium for 24 hrs and then treated with vehicle or PDGF-BB for 24 hrs.
Growth protocol The hAoSMCs were purchased from ScienCell and cultured in Smooth Muscle Cell Medium supplemented with 2% FBS and smooth muscle growth supplement in a humidified 5% CO2 incubator. hAoSMCs used for GeneChips detection were passage 5.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Affymetrix Scanner 3000 (Affymetrix).
Description Gene expression data from hAoSMCs treated with vehicle 24h
Data processing The data were analyzed with RMA using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
 
Submission date Oct 21, 2021
Last update date Oct 22, 2023
Contact name Xian Guo
E-mail(s) [email protected]
Phone 18321781291
Organization name Changhai hospital
Street address changhai road 168
City shanghai
ZIP/Postal code 200433
Country China
 
Platform ID GPL23178
Series (1)
GSE186313 Microassay analysis of the lncRNAs expression profile in human aortic vascular smooth muscle cells(hAoSMCs) treated with PDGF-BB at 24 h.

Supplementary file Size Download File type/resource
GSM5643616_0.CEL.gz 8.4 Mb (ftp)(http) CEL
Processed data are available on Series record

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