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Status |
Public on Oct 22, 2023 |
Title |
hAoSMCs treated with vehicle 24h rep1 |
Sample type |
RNA |
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Source name |
hAoSMCs treated with vehicle 24h
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Organism |
Homo sapiens |
Characteristics |
sample type: cell
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Treatment protocol |
Human AoSMCs were seeded in 6-well plates at an appropriate concentration, when the cell density reached 60-70%, hAoVSMCs were starved with serum-free medium for 24 hrs and then treated with vehicle or PDGF-BB for 24 hrs.
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Growth protocol |
The hAoSMCs were purchased from ScienCell and cultured in Smooth Muscle Cell Medium supplemented with 2% FBS and smooth muscle growth supplement in a humidified 5% CO2 incubator. hAoSMCs used for GeneChips detection were passage 5.
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Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
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Label |
biotin
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Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
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Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
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Scan protocol |
GeneChips were scanned using the Affymetrix Scanner 3000 (Affymetrix).
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Description |
Gene expression data from hAoSMCs treated with vehicle 24h
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Data processing |
The data were analyzed with RMA using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
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Submission date |
Oct 21, 2021 |
Last update date |
Oct 22, 2023 |
Contact name |
Xian Guo |
E-mail(s) |
[email protected]
|
Phone |
18321781291
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Organization name |
Changhai hospital
|
Street address |
changhai road 168
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City |
shanghai |
ZIP/Postal code |
200433 |
Country |
China |
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Platform ID |
GPL23178 |
Series (1) |
GSE186313 |
Microassay analysis of the lncRNAs expression profile in human aortic vascular smooth muscle cells(hAoSMCs) treated with PDGF-BB at 24 h. |
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