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Status |
Public on Jul 12, 2010 |
Title |
T17-F |
Sample type |
RNA |
|
|
Source name |
MCF-7, tamoxifin resistant, fulvestrant treated
|
Organism |
Homo sapiens |
Characteristics |
cell line: MCF-7 cell type: cancer cell culture resistance: tamoxifin subline: 17 treatment: fulvestrant
|
Treatment protocol |
Fulvestrant (0.1 mM in ethanol = 1000X working stock solution) was added directly to cell culture (10 microliter for a 10-cm dish containing 10 ml medium). Cells were incubated for 48 hours, and their total RNA was isolated for transcriptomal analysis.
|
Growth protocol |
MCF-7/BUS cell culture was maintained in high-glucose, phenol red-free DMEM (PF-DMEM) and supplemented with 5% fetal calf serum.
|
Extracted molecule |
total RNA |
Extraction protocol |
QIAGEN RNeasy protocol and reagents for extraction of total RNA
|
Label |
biotin
|
Label protocol |
Affymetrix One-Cycle Target Labeling and Control Reagents (Part#900493) + Enzo BioArray High Yield RNA Transcript Labelling Kit (Part#42655)
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|
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Hybridization protocol |
Microarray = Affymetrix GeneChip Human Genome U133 Plus 2.0. Affymetrix Hybridization Oven= 645. Affymetrix Fluidics Station= 450. Fluidics Protocol= EukGE-WS2
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Scan protocol |
Affymetrix GeneChip Scanner= 3000-7G. Controller= GCOS
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Description |
fulvestrant treated, tamoxifin resistant MCF-7 sub-line 17
|
Data processing |
MAS5 algorithm as implemented by call.exprs function of Bioconductor simpleaffy package version 2.14.05, 2% trimmed mean set to 100
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|
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Submission date |
Jul 12, 2010 |
Last update date |
Jul 12, 2010 |
Contact name |
Ben S. Wittner |
E-mail(s) |
[email protected]
|
Organization name |
Massachusetts General Hospital
|
Department |
Center for Cancer Research
|
Lab |
Lawrence
|
Street address |
149 13th Street
|
City |
Boston |
State/province |
MA |
ZIP/Postal code |
02129 |
Country |
USA |
|
|
Platform ID |
GPL570 |
Series (1) |
GSE14986 |
Antiestrogen-resistant subclones of MCF-7 human breast cancer cells are derived from a common clonal drug-resistant progenitor |
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