NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM565696 Query DataSets for GSM565696
Status Public on Jul 12, 2010
Title T17-F
Sample type RNA
 
Source name MCF-7, tamoxifin resistant, fulvestrant treated
Organism Homo sapiens
Characteristics cell line: MCF-7
cell type: cancer cell culture
resistance: tamoxifin
subline: 17
treatment: fulvestrant
Treatment protocol Fulvestrant (0.1 mM in ethanol = 1000X working stock solution) was added directly to cell culture (10 microliter for a 10-cm dish containing 10 ml medium). Cells were incubated for 48 hours, and their total RNA was isolated for transcriptomal analysis.
Growth protocol MCF-7/BUS cell culture was maintained in high-glucose, phenol red-free DMEM (PF-DMEM) and supplemented with 5% fetal calf serum.
Extracted molecule total RNA
Extraction protocol QIAGEN RNeasy protocol and reagents for extraction of total RNA
Label biotin
Label protocol Affymetrix One-Cycle Target Labeling and Control Reagents (Part#900493) + Enzo BioArray High Yield RNA Transcript Labelling Kit (Part#42655)
 
Hybridization protocol Microarray = Affymetrix GeneChip Human Genome U133 Plus 2.0. Affymetrix Hybridization Oven= 645. Affymetrix Fluidics Station= 450. Fluidics Protocol= EukGE-WS2
Scan protocol Affymetrix GeneChip Scanner= 3000-7G. Controller= GCOS
Description fulvestrant treated, tamoxifin resistant MCF-7 sub-line 17
Data processing MAS5 algorithm as implemented by call.exprs function of Bioconductor simpleaffy package version 2.14.05, 2% trimmed mean set to 100
 
Submission date Jul 12, 2010
Last update date Jul 12, 2010
Contact name Ben S. Wittner
E-mail(s) [email protected]
Organization name Massachusetts General Hospital
Department Center for Cancer Research
Lab Lawrence
Street address 149 13th Street
City Boston
State/province MA
ZIP/Postal code 02129
Country USA
 
Platform ID GPL570
Series (1)
GSE14986 Antiestrogen-resistant subclones of MCF-7 human breast cancer cells are derived from a common clonal drug-resistant progenitor

Data table header descriptions
ID_REF
VALUE MAS5 signal intensity

Data table
ID_REF VALUE
1007_s_at 1665.303749
1053_at 162.9776716
117_at 59.07036309
121_at 331.2213948
1255_g_at 6.762781771
1294_at 113.3907111
1316_at 28.22171089
1320_at 44.8686075
1405_i_at 2.816233166
1431_at 12.38906196
1438_at 156.249229
1487_at 264.3836941
1494_f_at 55.15696659
1552256_a_at 1087.481969
1552257_a_at 513.729305
1552258_at 26.49631895
1552261_at 20.04491414
1552263_at 25.39472601
1552264_a_at 233.5479402
1552266_at 2.382993013

Total number of rows: 54675

Table truncated, full table size 1212 Kbytes.




Supplementary file Size Download File type/resource
GSM565696.CEL.gz 5.4 Mb (ftp)(http) CEL
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap