|
| Status |
Public on May 31, 2022 |
| Title |
scRNA_HepG2 |
| Sample type |
SRA |
| |
|
| Source name |
liver cancer cell line
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: liver cancer cell line
cell line: HepG2
library strategy: scRNA-seq
|
| Growth protocol |
Cells were cultured in high glucose Dulbecco's modified Eagle's medium (DMEM) containing with 10% fetal bovine serum (FBS), 1% Penicillin-Streptomycin. 0.25% trypsin was used to digest cells.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
CITE-seq/scRNA-seq: Single cell was encapsulated into droplets and lysed in the droplet. mRNA and antibody-derived oligos were anneal to beads. After the reverse transcription procedure and PCR, the product was separated into ADTs and cDNA using Ampure Bead.
CITE-seq/scRNA-seq: DNBelab C Series High-throughput Single-cell System (BGI-research, China) was utilized for library preparation.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
DNBSEQ-T7 |
| |
|
| Data processing |
base-calling: PISA(V0.4)
alignment: PISA(V0.4)
filtering: PISA(V0.4)
cellCount: PISA(V0.4)
peak-calling: macs2
Genome_build: STAR
Supplementary_files_format_and_content: .txt
|
| |
|
| Submission date |
Nov 05, 2021 |
| Last update date |
Jun 01, 2022 |
| Contact name |
CNSA CNGB |
| Organization name |
BGI
|
| Street address |
BGI
|
| City |
shenzhen |
| ZIP/Postal code |
518083 |
| Country |
China |
| |
|
| Platform ID |
GPL29480 |
| Series (1) |
| GSE188289 |
Single-cell multi-omics dataset of hepatocellular carcinoma cell lines |
|
| Relations |
| BioSample |
SAMN22368103 |
| SRA |
SRX12667945 |
