|
Status |
Public on Jan 01, 2012 |
Title |
PBS Treated Cells 1 |
Sample type |
RNA |
|
|
Source name |
PBS Treated Cells 1
|
Organism |
Homo sapiens |
Characteristics |
cell line: Huh7.5.1 tissue: liver agent: PBS
|
Treatment protocol |
Ribavirin was added directly to the media and cells were expoed to drug for 24 hours at a concentration of 100 ug/mL
|
Growth protocol |
Cells were grown in 12 well dishes in DMEM, 10% FBS, 1% Antibiotic. 150,000 cells were seeded.
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted using the Quiagen RNeasy RNA isolation kit (Valencia, CA)
|
Label |
biotin
|
Label protocol |
Affimetrix standard label protocol
|
|
|
Hybridization protocol |
Affimetrix standard hyb protocol
|
Scan protocol |
Affimetrix standard scan protocol
|
Description |
1a
|
Data processing |
RMA by software Parteck
|
|
|
Submission date |
Jul 20, 2010 |
Last update date |
Jan 01, 2012 |
Contact name |
WeiPing Chen |
E-mail(s) |
[email protected]
|
Phone |
301-496-0175
|
Organization name |
NIDDK/NIH
|
Department |
GCL
|
Lab |
Genomics Core Lab
|
Street address |
Bldg 8, Room 1A11, NIDDK/NIH
|
City |
Bethesda |
State/province |
MD |
ZIP/Postal code |
20892 |
Country |
USA |
|
|
Platform ID |
GPL570 |
Series (1) |
GSE23031 |
Ribavirin Treated Huh7.5.1 Cells |
|