|
| Status |
Public on Jan 01, 2012 |
| Title |
PBS Treated Cells 2 |
| Sample type |
RNA |
| |
|
| Source name |
PBS Treated Cells 2
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: Huh7.5.1
tissue: liver
agent: PBS
|
| Treatment protocol |
Ribavirin was added directly to the media and cells were expoed to drug for 24 hours at a concentration of 100 ug/mL
|
| Growth protocol |
Cells were grown in 12 well dishes in DMEM, 10% FBS, 1% Antibiotic. 150,000 cells were seeded.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using the Quiagen RNeasy RNA isolation kit (Valencia, CA)
|
| Label |
biotin
|
| Label protocol |
Affimetrix standard label protocol
|
| |
|
| Hybridization protocol |
Affimetrix standard hyb protocol
|
| Scan protocol |
Affimetrix standard scan protocol
|
| Description |
1b
|
| Data processing |
RMA by software Parteck
|
| |
|
| Submission date |
Jul 20, 2010 |
| Last update date |
Jan 01, 2012 |
| Contact name |
WeiPing Chen |
| E-mail(s) |
[email protected]
|
| Phone |
301-496-0175
|
| Organization name |
NIDDK/NIH
|
| Department |
GCL
|
| Lab |
Genomics Core Lab
|
| Street address |
Bldg 8, Room 1A11, NIDDK/NIH
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE23031 |
Ribavirin Treated Huh7.5.1 Cells |
|
