 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Jan 03, 2022 |
| Title |
Mog-TDP43 Control A66-1 |
| Sample type |
SRA |
| |
|
| Source name |
Oligodendrocytes
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Cortex
age: P28
Sex: F
|
| Extracted molecule |
total RNA |
| Extraction protocol |
QIAGEN RNeasy micro kit
NuGEN Ovation single-cell RNAseq kit
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Description |
Mog-TDP43 Control
|
| Data processing |
Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence, then mapped to mm10 refSeq using the STAR spliced read aligner
Fragment counts were derived using HTS-seq program using mm10 Ensembl transcripts as model.
Differentially expressed genes were identified using edgeR which were then considered and ranked based on adjusted p-values (FDR) of ≤ 0.1.
Genome_build: mm10
Supplementary_files_format_and_content: Excel file including raw counts, normalized counts, and FPKM for each samples as well as DEG analysis between each condition
|
| |
|
| Submission date |
Nov 15, 2021 |
| Last update date |
Jan 03, 2022 |
| Contact name |
Dwight Bergles |
| E-mail(s) |
[email protected]
|
| Organization name |
Johns Hopkins University
|
| Department |
Neuroscience
|
| Lab |
Dwight Bergles
|
| Street address |
725 North Wolfe St., WBSB 1001
|
| City |
Baltimore |
| State/province |
MD |
| ZIP/Postal code |
21205 |
| Country |
USA |
| |
|
| Platform ID |
GPL19057 |
| Series (1) |
| GSE188903 |
Stage-specific control of oligodendrocyte survival and morphogenesis by TDP-43 |
|
| Relations |
| BioSample |
SAMN23168617 |
| SRA |
SRX13144563 |
| Supplementary data files not provided |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
|
|
|
|
 |
