|
| Status |
Public on Dec 04, 2021 |
| Title |
Unstimulaetd eosinophils e |
| Sample type |
SRA |
| |
|
| Source name |
Eosinophils
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
treatment: unstimulated
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The CEL-Seq library was ran on an Illumina NextiSeq550 instrument. The RNASeq experiment ran on the HISEQ platform
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
NextSeq 550 |
| |
|
| Data processing |
RNA-Seq data was trimmed using fastp 0.20.1
RNA-Seq data was aligned using STAR 2.7.2a. CEL-Seq data was aligned using Tophat2 version 2.1.0
CEL-Seq transcript quantification was performed using HTSeq-count 0.6.1 on union mode. RNA-Seq quantification was performed by STAR 2.7.2a.
Genome_build: GRCm38
Supplementary_files_format_and_content: tab-delimited text files include raw gene abundance for each sample
|
| |
|
| Submission date |
Nov 19, 2021 |
| Last update date |
Dec 04, 2021 |
| Contact name |
Guy Shapira |
| E-mail(s) |
[email protected]
|
| Organization name |
Tel Aviv University
|
| Street address |
Chaim Levanon 30
|
| City |
Tel Aviv |
| ZIP/Postal code |
6997801 |
| Country |
Israel |
| |
|
| Platform ID |
GPL21626 |
| Series (1) |
| GSE189213 |
Transcriptional profiling of mouse eosinophils identifies distinct gene signatures following cellular activation |
|
| Relations |
| BioSample |
SAMN23310716 |
| SRA |
SRX13179865 |
