|
| Status |
Public on Jun 08, 2022 |
| Title |
5_1250_KO |
| Sample type |
SRA |
| |
|
| Source name |
lung_AT2
|
| Organism |
Mus musculus |
| Characteristics |
age: Adult
genotype: SftpcCreERT2+/-;Klf5 fl/fl; eYFP-/-
strain: C57BL/6 CD1 mixed
Sex: male
treatment: Hyperoxia
|
| Growth protocol |
Lungs were homogenized by a combination of physical and enzymatic digestion before lineage traced AT2 cells were isolated by fluorescence activated cell sorting.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA-seq standard protocol
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Data processing |
Fastq files were aligned to the GRCm39 reference genome using STAR and default parameters
Ensmebl v04 gene annotations were used for gene counts, gene counts were obtain using STAR
Raw gene counts were transformed using 'LIMMA-Voom' method
Genome_build: mm9
Supplementary_files_format_and_content: Voom transformed abundance measurements
|
| |
|
| Submission date |
Dec 10, 2021 |
| Last update date |
Jun 09, 2022 |
| Contact name |
Michael Patrick Morley |
| E-mail(s) |
[email protected]
|
| Phone |
215-898-2026
|
| Organization name |
Perelman School of Medicine at the University of Pennsylvania
|
| Department |
Penn Cardiovascular Institute
|
| Street address |
3400 Civic Center Blvd, Bldg 421
|
| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19104 |
| Country |
USA |
| |
|
| Platform ID |
GPL24247 |
| Series (1) |
| GSE190676 |
Klf5 promotes alveolar epithelial type 1 cell lineage commitment during lung development and regeneration |
|
| Relations |
| BioSample |
SAMN23891725 |
| SRA |
SRX13377639 |
