|
| Status |
Public on Oct 18, 2010 |
| Title |
CrebA sorted 3 |
| Sample type |
RNA |
| |
|
| Source name |
St 11-15 embryos
|
| Organism |
Drosophila melanogaster |
| Characteristics |
developmental stage: embryo
genotype/variation: CrebA null mutant
|
| Treatment protocol |
Embryos were sorted on a COPAS biosorter prior to RNA extraction.
|
| Growth protocol |
Embryos were collected for eight hours and aged an additional 11 hours by the time of RNA extraction.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated by TriZol extraction and cleaned up using the Qiagen Rneasy kit.
|
| Label |
biotin
|
| Label protocol |
Amplification and labeling were performed using the Affymetrix GeneChip 3' IVT Express kit (P/N 901229) following the manufacturer's protocol.
|
| |
|
| Hybridization protocol |
Samples and controls were prepared using the Affymetrix GeneChip hybridization, wash and stain kit (P/N 900720). Arrays were prehybridized for 10 minutes prior to sample addition. Following sample addition, arrays were hybridized for 16 hours at 45 Celsius, and shaken at 60 rpm. Arrays were stained and washed using the Affymetrix GeneChip Fluidics Station 450 and FS450_002 fluidics script.
|
| Scan protocol |
Arrays were scanned with the Affymetrix GeneChip Scanner 3000 and raw analysis was performed with Affymetrix Command Console and Expression Console.
|
| Description |
CrebA mutant embryos st. 11-17 isolated based on lack of GFP balancer expression, sorted using a COPAS biosorter
|
| Data processing |
Data was normalized by RMA and statistical analysis was performed using Partek software. Further analysis was performed using Spotfire software.
|
| |
|
| Submission date |
Jul 30, 2010 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Deborah J Andrew |
| E-mail(s) |
[email protected]
|
| Organization name |
Johns Hopkins University School of Medicine
|
| Department |
Cell Biology
|
| Street address |
725 N. Wolfe St., Hunterian G-1
|
| City |
Baltimore |
| State/province |
MD |
| ZIP/Postal code |
21205 |
| Country |
USA |
| |
|
| Platform ID |
GPL1322 |
| Series (2) |
| GSE23346 |
CrebA is a major and direct regulator of secretory pathway gene expression |
| GSE23349 |
The CrebA/Creb3-like transcription factors are major and direct regulators of secretory capacity |
|
| Relations |
| Reanalyzed by |
GSE119084 |
