|
| Status |
Public on Jul 31, 2010 |
| Title |
W1118, rep 2 |
| Sample type |
RNA |
| |
|
| Source name |
Drosophila w1118 0 hour prepupa
|
| Organism |
Drosophila melanogaster |
| Characteristics |
tissue: whole body
developmental stage: prepupa
background strain: w1118
sample type: control
|
| Treatment protocol |
N/A
|
| Growth protocol |
Animals were raised at 25C on standard Drosophila media.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using TriPure (Roche), followed by purification with RNAeasy columns (Qiagen).
|
| Label |
biotin
|
| Label protocol |
Biotinylated aRNA were prepared according to the standard Affymetrix protocol from 0.5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix). The manual that was used has a copyright of 2008-2009.
|
| |
|
| Hybridization protocol |
Following fragmentation, 5 ug of aRNA were hybridized for 16 hr at 45C on the Drosophila Genome 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
|
| Description |
Gene expression data from 0 hour w1118 prepupa.
|
| Data processing |
The data were analyzed within Expression Console 1.1 using Affymetrix default analysis settings and RMA as the normalization method. Differential expression was assessed using Significance Analysis of Microarray (SAM).
|
| |
|
| Submission date |
Jul 30, 2010 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Robert B Beckstead |
| E-mail(s) |
[email protected]
|
| Phone |
706-542-0387
|
| Fax |
706-542-1827
|
| Organization name |
The University of Georgia
|
| Department |
Poultry Science
|
| Lab |
Robert B. Beckstead
|
| Street address |
119 Poultry Science Building
|
| City |
Athens |
| State/province |
GA |
| ZIP/Postal code |
30602-2772 |
| Country |
USA |
| |
|
| Platform ID |
GPL1322 |
| Series (1) |
| GSE23355 |
Expression profile for Drosophila Sox14 at onset of metamorphosis |
|
| Relations |
| Reanalyzed by |
GSE119084 |
