|
| Status |
Public on Sep 01, 2010 |
| Title |
AE25N |
| Sample type |
RNA |
| |
|
| Source name |
adjacent normal tissue
|
| Organism |
Homo sapiens |
| Characteristics |
tissue type: esophageal squamous cell carcinoma (ESCC)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted and purified by standard Trizol method and followed with qualification check by Agilent 2100.
|
| Label |
Biotin
|
| Label protocol |
As per manufacturer (Affymetrix).
|
| |
|
| Hybridization protocol |
Total RNA was reverse transcripted to cDNA, reverse transcripted to labelled aRNA, purified, fragmented, and hybridized to each array according to the manufacturer's instructions.
|
| Scan protocol |
The arrays were then washed using Affymetrix fluidics stations, and scanned using the Affymetrix GeneChip Scanner 3000.
|
| Description |
frozen tissue extracted by Trizol
|
| Data processing |
Microarray data were normalized using the R function rma() in the Bioconductor package affy
|
| |
|
| Submission date |
Aug 03, 2010 |
| Last update date |
Aug 03, 2010 |
| Contact name |
Howard Yang |
| E-mail(s) |
[email protected]
|
| Phone |
2402765257
|
| Organization name |
NCI
|
| Street address |
9609 Medical Center Dr.
|
| City |
Rockville |
| State/province |
MD |
| ZIP/Postal code |
20850 |
| Country |
USA |
| |
|
| Platform ID |
GPL96 |
| Series (1) |
| GSE23400 |
Global gene expression profiling and validation in esophageal squamous cell carcinoma (ESCC) |
|
