|
| Status |
Public on Aug 11, 2010 |
| Title |
macrophage_rIAPP15uM_12h_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
mouse bone marrow-derived macrophage
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
media: DMEM with 10% FBS
treatment: rat IAPP
cell type: bone marrow-derived macrophage
|
| Treatment protocol |
Cells were treated with 15 mM rat or human IAPP dissolved in media, and incubated for 12 hours at 37 degrees C.
|
| Growth protocol |
BMDMs were propagated from bone marrow in complete DMEM containing 15% L929-conditioned media for 7 days. Cells were seeded in 24-well plates and starved of L929-containing media for 12 hours prior to the experiment.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Buffer RLT provided in a QIAGEN RNeasy Plus mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with an Agilent Bioanalyser.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
Rat IAPP, Replicate 2
|
| Data processing |
The data were normalized by the rank-invariant method in Bead Studio
|
| |
|
| Submission date |
Aug 10, 2010 |
| Last update date |
Aug 10, 2010 |
| Contact name |
Clara Westwell-Roper |
| E-mail(s) |
[email protected]
|
| Phone |
604-875-3792
|
| Organization name |
Child and Family Research Institute, University of British Columbia
|
| Department |
Pathology and Laboratory Medicine
|
| Lab |
Dr. C. Bruce Verchere
|
| Street address |
950 West 28th Ave
|
| City |
Vancouver |
| State/province |
BC |
| ZIP/Postal code |
V5Z 4H4 |
| Country |
Canada |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE23534 |
Effect of hIAPP aggregation on gene expression by mouse bone marrow-derived macrophages after 12 hours |
|
