|
| Status |
Public on Feb 08, 2022 |
| Title |
Sir3myc_DSSY692_sil_Rep1 |
| Sample type |
SRA |
| |
|
| Source name |
haploid cells
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
strain: DSSY692/JRY13127
hm locus: HMLa::RFP
chip antibody: anti-myc
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Bead beating > Sonication > Clarification of lysate > ChIP with indicated antibody
NEB Ultra II kit
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina MiniSeq |
| |
|
| Data processing |
Reads aligned to S288C reference genome (GenBank accession number GCA_000146045.2) using bowtie2
Coverage was computed with a custom Python script (Goodnight & Rine, 2021)
Coverage was normalized to the non-heterochromatin genome-wide median using a custom Python script (Goodnight & Rine, 2021)
Coverage was smoothed with a 300bp rolling mean in R
Genome_build: S288C (GenBank accession number GCA_000146045.2)
Supplementary_files_format_and_content: bedgraph
|
| |
|
| Submission date |
Feb 01, 2022 |
| Last update date |
Feb 09, 2022 |
| Contact name |
Daniel S Saxton |
| E-mail(s) |
[email protected]
|
| Phone |
8314190756
|
| Organization name |
University of California Berkeley
|
| Department |
MCB
|
| Lab |
Rine
|
| Street address |
100 University Ave
|
| City |
Berkeley |
| State/province |
CA |
| ZIP/Postal code |
94709 |
| Country |
USA |
| |
|
| Platform ID |
GPL22715 |
| Series (1) |
| GSE195880 |
Distinct silencer states determine epigenetic states of heterochromatin |
|
| Relations |
| BioSample |
SAMN25555274 |
| SRA |
SRX14006145 |
