|
| Status |
Public on Feb 01, 2011 |
| Title |
Control A549 cells for aged exposure 2 |
| Sample type |
RNA |
| |
|
| Source name |
A549 cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: A549 cells
treatment group: aged exposure
|
| Treatment protocol |
Cells at air-liquid interface exposed to humidified air for 4 hrs
|
| Growth protocol |
A549 cells were grown in F-12K media
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen RNeasy Kit
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol
|
| |
|
| Hybridization protocol |
Following fragmentation, cRNA was hybridized on Affymetrix Human Gene 1.0 ST array
|
| Scan protocol |
GeneChips were scanned using Affymetrix scanner
|
| Description |
Gene expression data from untreated lung cells
|
| Data processing |
Data were analyzed with Robust multichip analysis in Partek version 6.4.
|
| |
|
| Submission date |
Aug 20, 2010 |
| Last update date |
Nov 13, 2013 |
| Contact name |
Rebecca Catherine Fry |
| E-mail(s) |
[email protected]
|
| Organization name |
UNC-Chapel Hill
|
| Department |
Environmental Sciences and Engineering
|
| Street address |
1213 MHRC
|
| City |
Chapel Hill |
| ZIP/Postal code |
27599 |
| Country |
USA |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE23735 |
mRNA expression data from A549 exposed to fresh or aged urban air mixtures |
|
