|
| Status |
Public on Sep 23, 2010 |
| Title |
AR66Q E897K, vehicle-treated, replicate 3 |
| Sample type |
RNA |
| |
|
| Source name |
Head expressing AR66Q E897K mutant, treated with ethanol
|
| Organism |
Drosophila melanogaster |
| Characteristics |
strain: GMR-GAL4 x UAS-AR
androgen receptor (ar) status: 66 polyglutamine, E897K mutation
sex: female
age: 5 days
developmental stage: adult
tissue: head
treatment: ethanol
|
| Treatment protocol |
Flies were bred on food containing either 1mM DHT or 1% ethanol (vehicle).
|
| Growth protocol |
UAS-AR flies were crossed to GMR-GAL4 flies at 29°C on food containing either 1 mM DHT (Steraloids) or 1% ethanol (vehicle).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Heads of 15 female offspring were collected and frozen, and RNA was extracted using TRIzol (Invitrogen).
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA was prepared according to the Affymetrix 3' IVT Express protocol from 100 ng total RNA (Affymetrix Technical Manual, 2008).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on the Affymetrix Drosophila Genome 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
|
| Description |
nbn019
Experiment 1
|
| Data processing |
The data were analyzed with GeneChip Operating Software version 1.4 using the default MAS 5.0 algorithm settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
|
| |
|
| Submission date |
Aug 25, 2010 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Geoffrey Neale |
| E-mail(s) |
[email protected]
|
| Organization name |
St Jude Childrens Research Hospital
|
| Department |
Hartwell Center
|
| Street address |
262 Danny Thomas Place
|
| City |
Memphis |
| State/province |
TN |
| ZIP/Postal code |
38105 |
| Country |
USA |
| |
|
| Platform ID |
GPL1322 |
| Series (1) |
| GSE23802 |
Native functions of the androgen receptor are essential to pathogenesis in a Drosophila model of spinobulbar muscular atrophy |
|
| Relations |
| Reanalyzed by |
GSE119084 |
