|
| Status |
Public on Feb 12, 2011 |
| Title |
conventional glioma cell line, serum, est. from GS line-generating tumors_ML-8 p8 |
| Sample type |
RNA |
| |
|
| Source name |
conventional glioma cell line established from primary tumor GS.8T
|
| Organism |
Homo sapiens |
| Characteristics |
sample name: ML-8 p8
sample type: conventional glioma cell line (monolayer culture established from the same tumors as GS-lines using standard serum conditions)
|
| Growth protocol |
conventional glioma cell lines and self established glioma cell lines (ML lines) -->Dulbecco´s modified Eagle´s medium, supplemented with 10% fetal calf serum, 2 mM L-glutamine, and 1 mM sodium pyruvate / glioblastoma stem-like cell lines (GS lines) --> neurobasal medium with B27 supplement (20 μl ml−1), Glutamax (10 μl ml−1), fibroblast growth factor-2 (20 ng ml−1), epidermal growth factor (20 ng ml−1) and heparin (32 IE ml−1)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from cells using the RNeasy Protect Mini Kit (Qiagen). Genomic DNA contamination was removed through an on-column DNase digestion step.
|
| Label |
biotin
|
| Label protocol |
standard Affymetrix protocols
|
| |
|
| Hybridization protocol |
standard Affymetrix protocols
|
| Scan protocol |
standard Affymetrix protocols
|
| Description |
conventional glioma cell line, serum conditions, established from GS line-generating tumors
|
| Data processing |
background-adjusted, normalized intensities were computed using the gcRMA package. Gene detection was performed using the PANP package (http://bioconductor.org/packages/bioc/vignettes/panp/inst/doc/panp.pdf) for the R program.
|
| |
|
| Submission date |
Aug 25, 2010 |
| Last update date |
Feb 12, 2011 |
| Contact name |
Hauke Simon Guenther |
| Organization name |
UKE
|
| Department |
Neurochirurgie
|
| Lab |
Endolab2
|
| Street address |
Martinistraße 52
|
| City |
Hamburg |
| ZIP/Postal code |
20246 |
| Country |
Germany |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE23806 |
Expression data of glioblastoma stem-like (GS) cell lines, conventional glioma cell lines and primary tumors |
|
