|
| Status |
Public on Dec 31, 2010 |
| Title |
strain DSY717; PDR1 with substitution L1081F DSY717_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
DSY717_rep1
|
| Organism |
Nakaseomyces glabratus |
| Characteristics |
genotype: PDR1 with substitution L1081F
isolate: DSY717
|
| Growth protocol |
log-pase culture grown in YPD
|
| Extracted molecule |
total RNA |
| Extraction protocol |
total RNA extraction using glass beads
|
| Label |
cy3
|
| Label protocol |
Agilent one-color quick amp labeling kit protocol
|
| |
|
| Hybridization protocol |
Agilent gene expression hybridization kit protocol
|
| Scan protocol |
Agilent Scanning protocol on an Agilent DNA Microarray Scanner G2565BA Other: Arrays were scanned at 5um resolution and 100/10% PMT XDR
|
| Description |
strain DSY717; PDR1 with substitution L1081F
|
| Data processing |
Data were extracted from the images with Feature Extraction (FE) software. Data were normalized using a combined rank consistency filtering with LOWESS intensity normalization. The gene expression values obtained from FE software were imported into GeneSpring 10.0.2 software (Agilent technologies) for preprocessing and data analysis.
|
| |
|
| Submission date |
Aug 26, 2010 |
| Last update date |
Dec 31, 2010 |
| Contact name |
Dominique Sanglard |
| E-mail(s) |
[email protected]
|
| URL |
http://www.chuv.ch/imul
|
| Organization name |
University of Lausanne and University Hospital Center
|
| Street address |
Rue Bugnon 48
|
| City |
Lausanne |
| ZIP/Postal code |
1011 |
| Country |
Switzerland |
| |
|
| Platform ID |
GPL10713 |
| Series (2) |
| GSE23828 |
Contribution of CgPDR1-regulated genes in enhanced virulence of azole-resistant Candida glabrata (part 2) |
| GSE23829 |
Contribution of CgPDR1-regulated genes in enhanced virulence of azole-resistant Candida glabrata |
|
