|
| Status |
Public on Aug 28, 2010 |
| Title |
20wk UPII-SV40Tag biological rep #2 |
| Sample type |
RNA |
| |
|
| Source name |
Urothelial RNA, 20 wks of age
|
| Organism |
Mus musculus |
| Characteristics |
genetic background: FVB parent strain
genotype: Transgenic, uroplakin II promoter driving expression of SV40Tag
tissue: urothelium
age: 20wk
|
| Growth protocol |
WT and UPII-SV40Tag mice were sacrificed at 3, 6, 20, and 30 weeks of age and the bladders immediately removed. The urothelial lining was removed from the bladder for RNA extraction.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was purified using TriReagent according to manufacturers instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from approximately 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45°C on the Mouse 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
|
| Description |
Urothelial RNA, 20 wks of age
|
| Data processing |
Pixel intensities were measured, expression signals were analyzed and features extracted using GeneChip Operating Software 1.3. Global scaling with a target intensity of 500 was used for normalization.
|
| |
|
| Submission date |
Aug 27, 2010 |
| Last update date |
Aug 27, 2010 |
| Contact name |
John L Clifford |
| E-mail(s) |
[email protected]
|
| Phone |
318-675-8264
|
| Fax |
318-675-5180
|
| Organization name |
LSUHSC-Shreveport
|
| Department |
Biochemistry
|
| Lab |
Clifford
|
| Street address |
1501 Kings Hwy
|
| City |
Shreveport |
| State/province |
LA |
| ZIP/Postal code |
71106 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE23845 |
Time course for bladder UCC development in UPII-SV40Tag mice |
|
