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Status |
Public on Sep 03, 2010 |
Title |
Thymic tumor from mouse RJ28 |
Sample type |
RNA |
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|
Source name |
transformed T cells from a mouse thymic lymphoma
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Organism |
Mus musculus |
Characteristics |
age: 8-10 weeks disease state: lymphoma tissue: thymus cell type: transformed T cells genotype/variation: IkL/L Notch1f/f CD4-Cre+
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Treatment protocol |
Thymic lymphomas were dissected from euthanized sick mice and tumor cells collected after tissue disruption between frosted ends of microscope slides
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Extracted molecule |
total RNA |
Extraction protocol |
RNAs were extracted with the RNAeasy kit, following the manufacturer's instructions (including DNAse I treatment)
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
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|
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Hybridization protocol |
Following fragmentation, 12 ug of cRNA were hybridized for 16 hr at 45C on GeneChip 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
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Scan protocol |
Arrays were scanned with a GeneChip® Scanner 3000 7G System
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Description |
gene expression data from a thymic tumor from an Ikaros deficient thymic lymphoma with a Notch promoter/exon1 deletion
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Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. Target intensity was set at 100.
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Submission date |
Sep 03, 2010 |
Last update date |
Dec 05, 2013 |
Contact name |
Susan Chan |
E-mail(s) |
[email protected]
|
Phone |
33 3 88 65 34 72
|
Organization name |
IGBMC
|
Street address |
1, rue Laurent Fries
|
City |
Illkirch |
ZIP/Postal code |
67404 |
Country |
France |
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Platform ID |
GPL1261 |
Series (1) |
GSE23972 |
Ikaros deficient mouse thymic lymphomas |
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