|
| Status |
Public on Mar 10, 2022 |
| Title |
Total RNA from Quadriceps 10 degree cold mouse [Q4_S31] [re-used] |
| Sample type |
SRA |
| |
|
| Source name |
Quadriceps
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6J
tissue: Quadriceps
age: 12 weeks
genotype: wild type
treatment: CE
|
| Treatment protocol |
34 °C for 4 weeks
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol
TruSeq stranded mRNA kit
Tissues were removed, flash frozen on dry ice, and RNA was harvested using Trizol reagent. Illumina RNA Kits were used for the construction of sequencing libraries.
RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Data processing |
The reads were mapped with STAR aligner v.2.5.3a to the UCSC Mus musculus mm10
reference. The average mapping rate is 80 %.
The transcriptome metrics were evaluated with the Picard tools v.1.141
The table of counts with the number of reads mapping to each gene feature of the UCSC Mus
Genome_build: musculus mm10 was prepared with HTSeq v0.6p1
Supplementary_files_format_and_content: Matrix table with raw gene counts for every gene and every sample
|
| |
|
| Submission date |
Mar 07, 2022 |
| Last update date |
Mar 16, 2022 |
| Contact name |
Noushin Hadadi |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Geneva
|
| Department |
Physiology and Cellular Metabolism
|
| Street address |
1 rue Michel-Servet
|
| City |
Genève |
| ZIP/Postal code |
1211 |
| Country |
Switzerland |
| |
|
| Platform ID |
GPL13112 |
| Series (1) |
| GSE198046 |
Comparative multi-tissue profiling reveals extensive tissue-specificity in transcriptome reprogramming |
|
| Relations |
| BioSample |
SAMN26507280 |
| SRA |
SRX14398157 |
